Fluorescence Analysis for Multi-Site Aluminum Binding to Natural Organic Matter

Natural organic matter (NOM) samples isolated from different water sources in Norway were compared using their fluorescence properties. Fluorescence surfaces were observed at pH 4.36 and deconvoluted using SIMPLISMA (Windig and Guilment 1991). There were a total of seven different fluorophores observed for these samples and each sampling site had between four and six ofthe fluorescent components. These components were observed to bind Al during titrations at the same pH. Multiresponse titration curves were fit using the method of Smith and Kramer (1998) and most of the binding strengths are similar to values for Suwannee River fulvic acid (1ogK’ between 4.8 and 5.5), but there are strong sites (IogK’ = 7) and weak sites (1ogK’ between 3 and 4) also observed. Results depended on the isolation method used; reverse osmosis and low pressure evaporation yielded different values but with no consistent trends

Multinomial selection index

Comparison of multivariate statistical analysis techniques for multinomial selection indice

Characterization of Freshwater Natural Dissolved Organic Matter (DOM): Mechanistic Explanations for Protective Effects Against Metaltoxicity and Direct Effects on Organisms

Dissolved organic matter (DOM) exerts direct and indirect influences on aquatic organisms. In order to better understand how DOM causes these effects, potentiometric titration was carried out for a wide range of autochthonous and terrigenous freshwater DOM isolates. The isolates were previously characterized by absorbance and fluorescence spectroscopy. Proton binding constants (pKa) were grouped into three classes:acidic (pKa ≤ 5), intermediate (5 \u3c pKa ≤ 8.5) and basic (pKa \u3e 8.5). Generally, the proton site densities (LT) showed maximum peaks at the acidic and basic ends around pKa values of 3.5 and 10, respectively. More variably positioned peaks occurred in the intermediate pKa range. The acid–base titrations revealed the dominance of carboxylic and phenolic ligands with a trend for more autochthonous sources to have higher total LT. A summary parameter, referred to as the Proton Binding Index (PBI), was introduced to summarize chemical reactivity of DOMs based on the data of pKa and LT. Then, the already published spectroscopic data were explored and the specific absorbance coefficient at 340 nm (i.e. SAC340), an index of DOM aromaticity,was found to exhibit a strong correlation with PBI. Thus, the tendencies observed in the literature that darker organic matter is more protective against metal toxicity and more effective in altering physiological processes in aquatic organisms can now be rationalized on a basis of chemical reactivity to protons

The Influence of Dissolved Organic Matter (DOM) on Sodium Regulation and Nitrogenous Waste Excretion in the Zebrafish (Danio rerio)

Dissolved organic matter (DOM) is both ubiquitous and diverse in composition in natural waters, but its effects on the branchial physiology of aquatic organisms have received little attention relative to other variables (e.g. pH, hardness, salinity, alkalinity). Here, we investigated the effects of four chemically distinct DOM isolates (three natural, one commercial, ranging from autochthonous to highly allochthonous, all at ∼6 mg C l−1) on the physiology of gill ionoregulation and nitrogenous waste excretion in zebrafish acclimated to either circumneutral (7.0–8.0) or acidic pH (5.0). Overall, lower pH tended to increase net branchial ammonia excretion, net K+ loss and [3H]PEG-4000 clearance rates (indicators of transcellular and paracellular permeability, respectively). However, unidirectional Na+ efflux, urea excretion and drinking rates were unaffected. DOM sources tended to stimulate unidirectional Na+ influx rate and exerted subtle effects on the concentration-dependent kinetics of Na+ uptake, increasing maximum transport capacity. All DOM sources reduced passive Na+ efflux rates regardless of pH, but exerted negligible effects on nitrogenous waste excretion, drinking rate, net K+ loss or [3H]PEG4000 clearance, so the mechanism of Na+ loss reduction remains unclear. Overall, these actions appear beneficial to ionoregulatory homeostasis in zebrafish, and some may be related to physicochemical properties of the DOM sources. They are very different from those seen in a recent parallel study on Daphnia magna using the same DOM isolates, indicating that DOM actions may be both species and DOM specific

The Mars Global Dust Storm of 2018

Mars is a dusty planet. Wind often lifts dust from the surface into the air forming clouds of dust at different locations across Mars. These dust storms typically last up to a couple days and grow to a few hundred km in size. However, once in a long while when conditions are just right, localized dust storms can interact in a way that optically thick suspended dust covers nearly the entire planet remaining aloft for weeks to months. These global-scale dust storms are the most dramatic of all weather phenomena on Mars, greatly altering the thermal structure and dynamics of the Martian atmosphere and significantly changing the global distribution of surface dust. Such a global-scale dust storm occurred during the summer of 2018, the first such event since 2007. The global dust storm was observed by an international fleet of spacecraft in Mars orbit and on the surface of Mars providing an unprecedented view of the initiation, growth, and decay of the storm as well as the physical properties of the dust during the storm's evolution. The 2018 global-scale dust storm was observed to grow from several localized dust-lifting centers with wind-blown dust suspended in the atmosphere encircling Mars after about two weeks of activity. Dust column optical depths recorded by the Opportunity and Curiosity rovers on the surface were the highest ever recorded on Mars. Peak global intensity of the dust storm was reached in early July 2018. Over the next couple months, the dust settled out and the atmosphere returned to its climatological average. Only a small number of global-scale dust storms have been observed on Mars, and so detailed analysis of the observations of this storm will provide important new insight into how these events occur and their effect on the current Mars climate

IST\u27s DIS Pdu Flooder: Final Report

Report on distributed interactive simulation application which is able to place large numbers of syntactically and semantically correct distributed interactive simulation protocol data units on a network to simulate the traffic that would be generated by multiple distributed interactive simulation applications

The Prediction of Sediment and Nutrient Transport in the Buffalo River Watershed Using A Geographic Information System

The Buffalo River was established by Congress in 1972 as the first National River in the United States. It is one of the few remaining free-flowing streams in northern Arkansas. The river originates in the higher elevations of the Boston Mountains in Newton County, and generally flows northeastward, intersecting the Springfield and Salem Plateaus as it drops from approximately 2000 feet in the headwaters to around 500 feet at the confluence with the White River in Marion County. It is considered by many to be one of Arkansas\u27 greatest natural treasures, and therefore , there is strong interest in protecting it from undue influences of man. One of the best general descriptions of the area within the Buffalo River watershed was given by Smith (1967)